ABSTRACT
Abstract Chronic periodontitis (CP) is characterized by gingival inflammation and bone destruction. It has been reported that interferon-gamma (IFN-γ) levels are high in CP patients; however, the IFN-γ receptor (IFN-γR) has not been studied in gingival tissue from these patients. Objective: To evaluate IFN-γ levels and IFN-γR expression in gingival tissue biopsies from chronic periodontitis patients compared with healthy subjects (HS). Material and Methods: Gingival tissues were obtained from all study subjects, CP (n = 18) and healthy subjects (HS) (n = 12). A tissue section of each study subject was embedded in paraffin blocks to determine the expression of IFN-γ R (IFN-γR1 and IFN-γR2) through immunohistochemistry. Another section of the tissue was homogenized and IFN-γ was measured by the ELISA technique. Results: No significant differences were found in the IFN-γR1 expression within the cell layers of the gingival tissue of the study groups. When analyzing the IFN-γR2 expression it was found that IFN-γR2 is strongly expressed in the endothelial cells of CP patients when compared to HS (p<0.05). IFN-γ concentrations in the gingival tissue were significantly higher in CP patients than in HS. No significant correlation between IFN-γ levels and the expression of IFN-γR1 and IFN-γR2 was found. However, a positive correlation between IFN-γ levels and clinical parameters [probing depth (PD) and clinical attachment level (CAL)] was found. Conclusion: The study of IFN-γR expression in gingival tissue samples from patients with CP showed an increase only in the IFN-γR2 chain in endothelial cells when compared to HS.
Subject(s)
Humans , Male , Female , Adult , Interferon-gamma/analysis , Receptors, Interferon/analysis , Endothelial Cells/pathology , Chronic Periodontitis/pathology , Gingiva/pathology , Reference Values , Biopsy , Enzyme-Linked Immunosorbent Assay , Immunohistochemistry , Case-Control Studies , Statistics, Nonparametric , Middle AgedABSTRACT
El mixoma cardíaco ha sido un reto para el cardiólogo, ya que sus manifestaciones clínicas pueden, no infrecuentemente, sugerir una lesión valvular. En el corazón es la neoplasia más frecuente, constituyendo el 50% de los casos de tumor intracardíaco. En este trabajo se presentan los aspectos morfológicos de 17 pacientes con diagnóstico de mixoma auricular. En 14 de los 17 casos la localización fue en la aurícula izquierda. En todos ellos el tumor estaba constituído por mucosubstancias y nidos celulares con células de aspecto estrellado. En base a los hallazgos, consideramos que el mixoma es una neoplasia cardiaca y no un trombo como se ha postulado en la literatura. Creemos que la célula de origen de este tumor es una célula pluripotencial, sobre la cual, aún quedan incógnitas por dilucidar